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Cytodétection des phycotoxines diarrhéiques - Une alternative au test de toxicité aiguë sur souris pour la surveillance de la salubrité des coquillages ArchiMer
Amzil, Zouher.
The cytodetection test of diarrheic phycotoxin KB-cells (DRAME test) was developed to ultimately provide a substitute for the acute toxicity mouse-test currently used by IFREMER's phytoplankton monitoring network (REPHY). The cyctodetection test relies on the cell morphological changes induced by the phosphorylation of cytoskeleton proteins resulting from the inhibition of cell protein-phosphatases by okadaic-type toxins. The method is specific, sensitive and fast with results given on the same day. In addition, the method has been shown to produce a good correlation with the acute toxicity mouse-test as weil as with HPLC analyses of diarrheic phycotoxins. The cytodetection test was awarded the only Special Prize of the Jury at the 6th AMAL THEE Awards...
Tipo: Text Palavras-chave: Acide okadaïque; Inhibition des protéines phosphatases; Cytodétection; Cellules KB; Okadaic acid; Inhibition of protein-phosphatase; Cytodeteciton KB-cells.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00156/26730/24811.pdf
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